Thursday 26 April 2012

LAB 4: SOURCES OF CONTAMINATION AND INFECTION


LAB 4: YASMIN SYAFIKAH BT
         RAZALI  (111435)


INTRODUCTION

                Airborne microorganisms are usually carried on dust particles, although some (fungal spores, for example ) may be carried directly by air current. It is important for microbiologists to be aware of the potential for contamination by airborne microorganisms. Carefully observation of simple precautions dramatically reduces the risk of contamination of the cultures.

                 Every human is colonized by billions of microorganisms. These 
microorganisms, some of hitch are vital to be our wellbeing, constitute our resident or normal microflora. Resident microorganisms are nourished by the chemicals and moisture excreted by human body. In moist areas such as the armpits, there are may be one million bacteria per centimeter cube, on the drier skin of forearm, there may be 10000 bacteria per centimeter cube.

               Resident microorganisms are neither nonpathogenic or are prevented from infecting the body by an array of mechanical and chemical defenses. Some resident microbes are however opportunistic pathogens which may cause infection if the body’s defenses are breached for example if the skin is broken.

               Transient microorganisms are picked up from our environment, for example from faecal contact or from soil and usually fail to become permanent skin residents. One of the most important reasons for failure to gain permanence is that the established residents are better able to compete for nutrients. Since transient generally originate in other environment, they are poorly adapted to conditions on the skin and usually disappear within 24 hours of arrival.

                    Microorganisms in the upper respiratory tract are either normal residents or transients. As with the skin, the normal microflora largely consists of nonpathogens opportunistic pathogens.Large numbers of transients enter the upper respiratory tract as we breathe or eat. They may also come from our own hands or from improper sanitation during food preparation. Regardless of their origin, most transients are nonpathogenic and are quickly killed by various defenses arrayed against them.



OBJECTIVE

To determine the microorganisms in the air and from healthy human


RESULT

AIR




HANDS




EAR





NORMAL BREATHING






VIOLENT COUGHING






DISCUSSION

                  
                    Microbes are found everywhere, but they are mostly far too small to be seen by the naked eye. This activity allows students to discover that microbes are found in a range of different habitats, to explore the variety of microorganisms around us and to compare the range of microorganisms that are found in different places. The air we breathe is teeming with more than 1,800 kinds of bacteria, including harmless relatives of microbes. The bacteria that can be exist in the culture that is exposed to air is the Bacillus cereus. Bacillus  cereus causes two types of food poisoning in humans including diarrhoeal syndrome and emetic syndrome. Food poisoning results from its production of enterotoxins in the gastrointestinal tract. Bacillus species are aerobic, sporulating, rod-shaped bacteria which are ubiquitous in nature.


                    The wash water from our hand may contain Staphylococcus aureus. S. aureus can cause a range of illnesses, from minor skin infections, such as pimples,, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia. S. aureus reproduces asexually by binary fission. S. aureus can infect other tissues when barriers have been breached example skin or mucosal lining. This leads to furuncles and carbuncles. In infants, S. aureus infection can cause a severe disease such as staphylococcal scalded skin syndrome. In our ears maybe contain bacteria such as Staphylococcus epidermidis. S. epidermidis is not usually pathogenic, patients with compromised immune systems are often at risk for developing an infection. These infections can be both nosocomial or community acquired, but they pose a greater threat to hospital patients. This phenomenon may be the result of continuous use of antibiotics and disinfectants within hospitals, leading to evolutionary pressure toward more virulent strains of the organism. S. epidermidis causes biofilms to grow on plastic devices placed within the body. Infection can also occur in dialysis patients or anyone with an implanted plastic device that may have been contaminated. Another disease it causes is endocarditis. This occurs most often in patients with defective heart valves. In some other cases, sepsis can occur in hospital patients.


                       Normal breathing may contain bacteria named Streptococcus pneumonia because S. pneumonia is found normally in the upper respiratory tract, including the throat and nasal passages. S pneumoniae infection is also an important cause of sinusitis, septic arthritis, osteomyelitis, peritonitis, and endocarditis and an infrequent cause of other less-common diseases. S. pneumoniae is transmitted directly from person to person through close contact via respiratory droplets. The organism frequently colonizes the nasopharynx of healthy people, particularly young children, without causing illness. Transmission is thought to be common, but clinical illness occurs infrequently among casual contacts. If we having violent coughing the bacteria that exist is Haemophilus influenza. H. influenzae seems to occur in humans only. In infants and young children, H. influenzae type b (Hib) causes bacteremia, pneumonia, and acute bacterial meningitis. On occasion, it causes cellulitis, osteomyelitis, epiglottitis, and infectious arthritis.

CONCLUSION

                       Merely walking into a room can begin to stir up the microbial soup that fills it.  One person can send about 37 million bacteria into the air every hour, according to new research that measured microbes suspended in a room when it was occupied and unoccupied. We are constantly exposed to microorganisms. These microorganisms are classed as bacteria, viruses, mould and fungi and are present around us all of the time, are part of life's delicate balance and normally do not cause us harm. Airborne microbes cause a lot of illnesses and diseases in humans.  

                     Microorganisms can enter the air when a human or animal sneezes, or by the wind picking up the light particles and blowing them where humans are. When a human sneezes microorganisms leave the lungs at around 200 miles per hour. Some of the microorganisms that are growing in the mucus in the respiratory tract enter the air with the moisture particles that are sneezed out of the lungs. These microorganisms can be breathed into the lungs of another person and that person could get sick.

REFERENCES

      3.             http://microbewiki.kenyon.edu/index.php/Bacillus_cereus





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